Juan Qiao, Professor, Master's Supervisor. She obtained her Ph.D. in Analytical Chemistry from the Institute of Chemistry, Chinese Academy of Sciences in 2010. She served as Assistant Researcher/Associate Researcher at the Institute of Chemistry, Chinese Academy of Sciences from 2010 to 2022. Currently, she is a Professor in the Department of Pharmaceutical Engineering, School of New Materials and Chemical Engineering.

Her research focuses on the preparation of polymers and their applications in biopharmaceutical analysis. As a project leader, she has undertaken and completed 5 National and Beijing Natural Science Foundation projects. She has published 27 SCI papers as first author/corresponding author, applied for 2 patents, co-authored 2 monographs, and received 2 first-class Science and Technology Awards from the China Association for Instrumental Analysis.

I. Main Research Areas

1. Drug analysis by capillary electrophoresis
2. Preparation of temperature-sensitive fluorescent polymers and their application in intracellular temperature imaging
3. Preparation of targeted recognition/in-situ controllable polymer enzyme reactors and their application in proteolysis

II. Awards and Honors

1. Qi Li, Juan Qiao, Xiaoyu Mu, Nan Li, Yi Chen. Construction of Polymer Molecular Thermometers and Their Application in Cell Imaging, First Prize of Science and Technology Award, China Association for Instrumental Analysis, 2016.
2. Qi Li, Juan Qiao, Haizhi Zhang, Chunhe Yao, Ying Shen, Xiaoyu Mu, Rongyue Zhang, Peiyong Xin, Xiaoyi Wei, Yaping Li, Yi Chen. New Biochromatographic Methods Based on Protein and Chiral Amino Acid Analysis, First Prize of Science and Technology Award, China Association for Instrumental Analysis, 2012.

III. Undertaken Research Projects

1. National Natural Science Foundation General Project: Targeted Recognition of Cell Surface Glycoproteins and Their In-situ Controllable Enzymolysis (22074148), Project Leader.
2. National Natural Science Foundation Major Research Plan Cultivation Project: Study on Temperature and Neurotransmitter Changes During Emotional and Memory Processes (91732103), Project Leader.
3. National Natural Science Foundation General Project: Preparation of Stimuli-Responsive Polymer Porous Membrane Enzyme Reactors and Their Application in Proteolysis (21475137), Project Leader.
4. National Natural Science Foundation Youth Project: Preparation of Temperature-Sensitive Fluorescent Block Copolymers and Their Temperature-Sensitive Application in Cells (21205125), Project Leader.
5. Institute of Chemistry Cultivation Project: Preparation of Fluorescent Temperature-Sensitive Polymers and Study of Ca2+ Channel-Regulated Cell Temperature Changes (CMS-PY-201355), Project Leader.
6. Molecular Science Center Innovation Project: Study on Monolithic Column Enzyme Reactors and Amino Acid Metabolism by Microfluidic Chip Electrophoresis (CX-201023), Project Leader.

IV. Representative Academic Papers

1. Juan Qiao, Qian Ma, Xiangfei Li, Li Qi*. Redox-responsive polymer nanoreactors based on methionine sulfoxide for monitoring cell adhesion [J]. Analytical Chemistry, 2022, 94, 11807-11812.

2. Juan Qiao, Xiangfei Li, Li Qi*. Fluorescent polymer-modified gold nanobipyramids for temperature sensing during photothermal therapy in living cells [J]. Chinese Chemical Letters, 2022, 33, 3193-3196.

3. Juan Qiao, Yuying Song, Chuanfang Chen, Li Qi*. In situ determination of sialic acid on cell-surface with pH-regulated polymer enzyme nanoreactor [J]. Analytical Chemistry, 2021, 93, 7317-7322.

4. Juan Qiao, Deyu Wu, Yuying Song, Wenliang Ji, Qingwei Yue, Lanqun Mao, Li Qi*. Simultaneous monitoring of intracellular temperature and norepinephrine variation by fluorescent probes during norepinephrine reuptake [J]. Analytical Chemistry, 2021, 93, 14743-14747.

5. Juan Qiao, Lili Liu, Ji Shen, Li Qi*. Enzyme immobilization on a pH-responsive porous polymer membrane for enzymatic kinetics study [J]. Chinese Chemical Letters, 2021, 32, 3195-3198.

6. Juan Qiao, Li Qi*. Recent progress in plant-gold nanoparticles fabrication methods and bioapplications [J]. Talanta, 2021, 223, 121396.

7. Juan Qiao, Han Wu, Huan Wei, Lanqun Mao, Tie Wang, Li Qi*. Selective capture and in-situ controllable detection of D-glucose in cerebral systems [J]. Analytical Chemistry, 2020, 92, 4445-4450.

8. Juan Qiao, Yoon-Ho Hwang, Dong-Pyo Kim, Li Qi*. Simultaneous monitoring of temperature and Ca2+ concentration variation by fluorescent polymer during intracellular heat production [J]. Analytical Chemistry, 2020, 92, 8579-8583.

9. Juan Qiao, Junfang Jiang, Lili Liu, Ji Shen, Li Qi*. Enzyme reactor based on reversible pH controlled catalytic polymer porous membrane [J]. ACS Applied Materials & Interfaces, 2019, 11, 15133-15140.

10. Juan Qiao, Qianrong Liu, Han Wu, Huiwu Cai, Li Qi*. Non-enzymatic detection of serum glucose using a fluorescent nanopolymer probe [J]. Microchimica Acta, 2019, 186, 366(1-7).

11. Juan Qiao, Liping Zhao, Lili Liu, Li Qi*. L-Glutaminase enzyme reactor based on porous bamboo sticks and its application in enzyme inhibiters screening [J]. Talanta, 2019, 205, 120126(1-7).

12. Wenya Feng, Juan Qiao*, Dan Li, Li Qi*. Chiral ligand exchange capillary electrochromatography with dual ligands for enantioseparation of D,L-amino acids [J]. Talanta, 2019, 194, 430-436.

13. Juan Qiao, Chuanfang Chen, Dihua Shangguan, Xiaoyu Mu, Shutao Wang, Lei Jiang*, Li Qi*. Simultaneous monitoring of mitochondrial temperature and ATP fluctuation using fluorescent probes in living cells [J]. Analytical Chemistry, 2018, 90, 12553-12558.

V. Invention Patents

1. Qi Li, Ji Shen, Juan Qiao. Preparation Method of Stimuli-Responsive Polymer Matrix D-Amino Acid Oxidase Reactor and Its Enzymolysis Efficiency Regulation Method. Chinese Invention Patent, ZL202110463264.
2. Qi Li, Juan Qiao, Yaping Li, Xiaoyu Mu. An Immobilized L-Asparaginase Reactor. Chinese Invention Patent, ZL201210137793.2.

VI. Published Works

1. Wang Ke, Qiao Juan. "Optical Probes and Sensing Analysis" edited by Huimin Ma (Chapter 11), 2020, 448-472.
2. Juan Qiao, Li Qi. Design and preparation of stimuli-responsive AIE fluorescent polymers-based probes for cells imaging, Handbook of Aggregation, 2022.

VII. Recruitment Information

I am currently recruiting Master's students for the Bioengineering and Pharmacy (0860) professional degree (Pharmaceutical Engineering field 086002). Students with backgrounds in analytical chemistry, pharmaceutical analysis, etc. are welcome to join our team.